Liquid Chromatography‐Mass Spectrometry Analysis of DNA Polymerase Reaction Products
نویسندگان
چکیده
منابع مشابه
SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
متن کاملA Simple and Rapid Leaf Genomic DNA Extraction Method for Polymerase Chain Reaction Analysis
In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...
متن کاملAnalysis of DNA restriction fragments and polymerase chain reaction products by capillary electrophoresis.
Capillary electrophoresis (CE) is a new, high-resolution tool for the analysis of DNA restriction fragments and DNA amplified by the polymerase chain reaction (PCR). By combining many of the principles of traditional slab gel methods in a capillary format, it is possible to perform molecular size determinations of human and plant PCR amplification products and DNA restriction fragments. DNA res...
متن کاملMicrochip Electrophoresis and the Analysis of Polymerase Chain Reaction Products
and control provided by miniature electronic devices (7). However, DNA samples in biological fluids often are present at concentration levels that are too low for any direct test. Therefore, analysts normally use enzyme-induced amplification such as polymerase chain reaction (PCR) to increase the sample concentration before analysis (8,9). In this article, I will describe the use of microchip-b...
متن کاملParallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is. Here we describe an approach in which the first primer binds in a parallel complementary orientation to the single-stranded DNA, leading to synthesis in a parallel direction. Further reactions happened in a conven...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols in Nucleic Acid Chemistry
سال: 2011
ISSN: 1934-9270,1934-9289
DOI: 10.1002/0471142700.nc0716s47